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排序方式: 共有388条查询结果,搜索用时 109 毫秒
381.
Kengo Hamamura Naoya Matsunaga Eriko Ikeda Hideaki Kondo Hisako Ikeyama Kazutaka Tokushige Kazufumi Itcho Yoko Furuichi Yuya Yoshida Masaki Matsuda Kaori Yasuda Atsushi Doi Yoshifumi Yokota Toshiaki Amamoto Hironori Aramaki Yasuhiro Irino Satoru Koyanagi Shigehiro Ohdo 《The Journal of biological chemistry》2016,291(10):4913-4927
Chronic kidney disease (CKD) is associated with an increase in serum retinol; however, the underlying mechanisms of this disorder are poorly characterized. Here, we found that the alteration of hepatic metabolism induced the accumulation of serum retinol in 5/6 nephrectomy (5/6Nx) mice. The liver is the major organ responsible for retinol metabolism; accordingly, microarray analysis revealed that the hepatic expression of most CYP genes was changed in 5/6Nx mice. In addition, D-box-binding protein (DBP), which controls the expression of several CYP genes, was significantly decreased in these mice. Cyp3a11 and Cyp26a1, encoding key proteins in retinol metabolism, showed the greatest decrease in expression in 5/6Nx mice, a process mediated by the decreased expression of DBP. Furthermore, an increase of plasma transforming growth factor-β1 (TGF-β1) in 5/6Nx mice led to the decreased expression of the Dbp gene. Consistent with these findings, the alterations of retinol metabolism and renal dysfunction in 5/6Nx mice were ameliorated by administration of an anti-TGF-β1 antibody. We also show that the accumulation of serum retinol induced renal apoptosis in 5/6Nx mice fed a normal diet, whereas renal dysfunction was reduced in mice fed a retinol-free diet. These findings indicate that constitutive Dbp expression plays an important role in mediating hepatic dysfunction under CKD. Thus, the aggravation of renal dysfunction in patients with CKD might be prevented by a recovery of hepatic function, potentially through therapies targeting DBP and retinol. 相似文献
382.
Eric Capo Benjamin D. Peterson Minjae Kim Daniel S. Jones Silvia G. Acinas Marc Amyot Stefan Bertilsson Erik Björn Moritz Buck Claudia Cosio Dwayne A. Elias Cynthia Gilmour Marisol Goñi-Urriza Baohua Gu Heyu Lin Yu-Rong Liu Katherine McMahon John W. Moreau Jarone Pinhassi Mircea Podar Fernando Puente-Sánchez Pablo Sánchez Veronika Storck Yuya Tada Adrien Vigneron David A. Walsh Marine Vandewalle-Capo Andrea G. Bravo Caitlin M. Gionfriddo 《Molecular ecology resources》2023,23(1):190-204
Mercury (Hg) methylation genes (hgcAB) mediate the formation of the toxic methylmercury and have been identified from diverse environments, including freshwater and marine ecosystems, Arctic permafrost, forest and paddy soils, coal-ash amended sediments, chlor-alkali plants discharges and geothermal springs. Here we present the first attempt at a standardized protocol for the detection, identification and quantification of hgc genes from metagenomes. Our Hg-cycling microorganisms in aquatic and terrestrial ecosystems (Hg-MATE) database, a catalogue of hgc genes, provides the most accurate information to date on the taxonomic identity and functional/metabolic attributes of microorganisms responsible for Hg methylation in the environment. Furthermore, we introduce “marky-coco”, a ready-to-use bioinformatic pipeline based on de novo single-metagenome assembly, for easy and accurate characterization of hgc genes from environmental samples. We compared the recovery of hgc genes from environmental metagenomes using the marky-coco pipeline with an approach based on coassembly of multiple metagenomes. Our data show similar efficiency in both approaches for most environments except those with high diversity (i.e., paddy soils) for which a coassembly approach was preferred. Finally, we discuss the definition of true hgc genes and methods to normalize hgc gene counts from metagenomes. 相似文献
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Tsutomu Tajikawa Yuya Imamura Takaya Ohno Fumiya Muranishi Maki Kubota Kenkichi Ohba 《Journal of Biorheology》2013,27(1-2):1-8
This study tried to evaluate the deformability of each erythrocyte by measuring the time constant of shape recovery just after the erythrocytes left the microchannels. We fabricated a microchannel array with a 5μm-square, 100μm-long cross-section on a PDMS sheet. Three different kinds of blood samples were prepared—healthy erythrocytes as a control, artificially membrane-hardened erythrocytes and artificial hemoglobin solution-diluted erythrocytes—to investigate the influence of erythrocyte's mechanical property changes on the time constant of shape recovery. These shape recovery processes were modeled and analyzed by a standard liner solid model. As a result, the temporal variation of the compressive strain of all erythrocytes showed exponential decay with time elapsed like a first order lag system, so the time constant of shape recovery could be calculated from the semi-logarithmic relaxation curve. The stiffer the cell membrane was using glutaraldehyde, the shorter the time constant for relaxation became compared to healthy erythrocytes. The diluted hemoglobin erythrocytes snapped back quicker than healthy ones. In addition, the time constant of healthy blood drawn from females was clearly shorter than that collected from males. However, the time constant of fully hemoglobin substituted erythrocytes was not affected by gender difference. These results indicate that there is not a significant difference in the stiffness of healthy cell membranes regardless of individual and gender differences. On the other hand, the viscosity of the hemoglobin solution inside the cell is one of the significant factors affecting the time constant. Therefore, these results suggest that the deformability of individual erythrocytes can be quantitated by the time constant for relaxation measured by microchannel techniques. 相似文献
387.
Yuya Imamura Miwa Haruta Yusuke Tomita Keiko Matsumura Tokunori Ikeda Akira Yuno Masatoshi Hirayama Hideki Nakayama Hiroshi Mizuta Yasuharu Nishimura Satoru Senju 《PloS one》2016,11(4)
We previously reported a method to expand human monocytes through lentivirus-mediated introduction of cMYC and BMI1, and we named the monocyte-derived proliferating cells, CD14-ML. CD14-ML differentiated into functional DC (CD14-ML-DC) upon addition of IL-4, resulting in the generation of a large number of DC. One drawback of this method was the extensive donor-dependent variation in proliferation efficiency. In the current study, we found that introduction of BCL2 or LYL1 along with cMYC and BMI1 was beneficial. Using the improved method, we obtained CD14-ML from all samples, regardless of whether the donors were healthy individuals or cancer patients. In vitro stimulation of peripheral blood T cells with CD14-ML-DC that were loaded with cancer antigen-derived peptides led to the establishment of CD4+ and CD8+ T cell lines that recognized the peptides. Since CD14-ML was propagated for more than 1 month, we could readily conduct genetic modification experiments. To generate CD14-ML-DC that expressed antigenic proteins, we introduced lentiviral antigen-expression vectors and subjected the cells to 2 weeks of culture for drug-selection and expansion. The resulting antigen-expressing CD14-ML-DC successfully induced CD8+ T cell lines that were reactive to CMVpp65 or MART1/MelanA, suggesting an application in vaccination therapy. Thus, this improved method enables the generation of a sufficient number of DC for vaccination therapy from a small amount of peripheral blood from cancer patients. Information on T cell epitopes is not necessary in vaccination with cancer antigen-expressing CD14-ML-DC; therefore, all patients, irrespective of HLA type, will benefit from anti-cancer therapy based on this technology. 相似文献
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